Figure 8. Effect of heat-shock on HmNOS and HmHSP90 gene expression.

Competent larvae (14 hpf) were exposed to one of four heat-shock temperatures (29, 32 and 35°C) or retained at the control temperature (25°C) for 2 h. Samples for RNA were collected at the start of the experiment as larvae (14 hpf), at 2 h after the initiation of the heat shock (2 hpi) as either larvae (had not initiated metamorphosis) or post-larvae (had initiated metamorphosis), and at 24 h after the initiation of the heat shock (24 hpi) as either larvae (had not initiated metamorphosis) or post-larvae (had initiated metamorphosis). Transcript abundance was assessed by qRT-PCR using mRNA purified from pools of larvae or post-larvae. Data are presented as log-transformed mean ± SEM of three technical replicates.