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. 2013 Sep 3;8(9):e72816. doi: 10.1371/journal.pone.0072816

Figure 1. A. Basal activity of the human CYP27B1 promoter constructs (−1576 bp, −1170 bp, −926 bp, −789 bp, −409 bp, −200 bp) in HEK293 cells.

Figure 1

1α-hydroxylase promoter-driven firefly luciferase activity is normalized to renilla luciferase activity in each individual sample and expressed as relative luciferase activity units (RLU). *P<0.05 when compared to HEK293 cells transfected with empty vector. # P<0.05 when compared to HEK293 cells transfected with 1.6 kb CYP27B1 promoter construct. B. Effect of forskolin (10−5 M) on CYP27B1 promoter activity in HEK293 cells. Bars depict fold induction of relative luciferase activity expressed as mean±SEM. *P<0.05 when compared to HEK293 cells treated with vehicle for each promoter deletion construct. (n = 3 experiments, each performed in triplicate).