Figure 7. Transient assays of Phytophthora capsici RXLR, CRN, NLP and elicitin effectors in Nicotiana benthamiana.

(A) Pc118548 (NLP) and Pc508761 (elicitin) trigger cell death in N. benthamiana. The plant leaves were infiltrated with A. tumefaciens (strain GV3101) cells to express Pc22053 (RXLR), Pc506611 (CRN), Ps118548, Pc508761, P. infestans INF1 (positive control) or GFP (negative control). Photographs were taken 5 d after infiltration. (B) – (I), Pc22053 and Pc506611 suppress cell death caused by different cell death inducers in N. benthamiana. Agro-infiltration sites in each N. benthamiana leaf expressing Pc22053 (top left), Pc506611 (top right), or GFP (middle right) were challenged with A. tumefaciens expressing Bax (B), INF1 (C), Pc508761 (D), PsojNIP (E), Pc118548 (F), PsAvh241 (G), PsCRN63 (H) and Avr3a/R3a (I). The other three sites in each leaf expressing GFP only (middle left), inducer itself (bottom right) or infiltrated by 10 mM MgCl₂ buffer only (bottom left) served as controls. Photographs were taken 5 d after cell death inducer infiltration.