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. 2013 Sep 3;8(9):e74484. doi: 10.1371/journal.pone.0074484

Figure 5. GSK3β inhibitors increase the proliferation of the dental lamina.

Figure 5

(A–D) Immunofluorescence against phospho-Histone H3 (pH3) (red) in explants after 3 days of culture. DNA (Hoechst) is in blue. LiCl increases the number of pH3+ cells in the dental organ and the dental lamina appears wider in treated cultures (asterisk in B,D compared to A,C). (E, F) Quantification of the samples represented in A–D showing (E) increase of pH3+ cells and (F) increase in mitotic index after LiCl and BIO treatment, n = number of slices analyzed. (G) Distribution of the number of pH3+ cells along the different regions of the dental organ represented in the scheme. Significant differences were found in the dental lamina after LiCl and BIO treatment. Scale bar: 100 µm.