Figure 5. Cell-to-cell contact is not required for membrane transfer.

A. Effect on transfer of cell depleted supernatant, from PTxM-treated or untreated Green^−/Red⁺ Jurkat cells, added to a population of PTxM treated or untreated Green⁺/Red⁻ Jurkat cells with schematic representations of protocols below related data. Results represent the mean transfer population size of three independent assays with standard deviation. B. Effect on transfer of differentially centrifuged cell depleted supernatant from untreated Green−/Red+ Jurkat cells, added to a population of PTxM treated Green+/Red- Jurkat cells. Untreated Green+/Red- Jurkat cells with 200×g supernatant (grey field) and PTxM treated Green+/Red- Jurkat cells with 200×g supernatant (black solid line), 16,000×g supernatant (dark grey solid line), 100,000×g supernatant (dark grey dashed line), or the resuspended pellet from the100,000×g supernatant (black dashed line).