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. 2013 Sep 3;8(9):e72885. doi: 10.1371/journal.pone.0072885

Figure 8. Recombinant S2S4 and S3S4 PTx Dimers are Robust Activators of Signal Transduction in Jurkat cells.

Figure 8

Recombinant S2S4 and S3S4 PTx dimers were purified from E. coli and tested for their abilities to activate PLC and the MAP kinase ERK. S2S4 and S3S4 dimers containing mutations in the conserved carbohydrate binding domain (ΔSA-S2S4 and ΔSA-S3S4) were also similarly tested. A. Wild type Jurkat cells were stimulated with the indicated concentrations of dimers and PLC activity was measured using the inositol phosphate accumulation assay. B. Wildtype Jurkat cells were stimulated with the indicated concentrations of dimers and ERK activity was measured by western blot using phospho-specific ERK antibodies.