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. 2013 Sep 3;8(9):e74349. doi: 10.1371/journal.pone.0074349

Figure 8. Collagen and mineral distribution across femoral insertions (transverse PMMA-embedded samples).

Figure 8

A) Light microscopy images showing the scan area (bar = 200 μm). B) Confirming the analysis of the paraffin-embedded sections, high collagen content was again found in the ligament and bone regions, with a consistent decrease in collagen within the fibrocartilage interface progressing from ligament to bone. C) The mineral:matrix ratio was found to be high in the bone and mineralized fibrocartilage, with D) an abrupt change in mineral content between the non-mineralized fibrocartilage and mineralized fibrocartilage regions. Blue and red colors in the spectroscopic maps indicate low and high content, respectively (Blue and red lines represent mean values and standard deviation, respectively; n=3; NFC = Non-Mineralized Fibrocartilage, MFC = Mineralized Fibrocartilage).