Table 1. Primers used for MLST analysis of Asian C. neoformans var. grubii isolates.

Locus	Primer name	Primer sequence	Amplification conditions	Reference
CAP59	CAP59LF	5′ GTGAACAAGCTGCGGC 3′	96°C 5min; 35 cycles: 96°C 30s, 56°C 30s, 72°C 1min;	Hagen et al., 2012b;
	CAP59LR	5′ GGATTCAGTGTGGTGGAAGA 3′	72°C 5min	Fraser et al., 2005
GPD1	GPD1LF	5′ GGTTGTCAAGGTTGGAATCAACGG 3′	96°C 5min; 35 cycles: 96°C 30s, 61°C 30s, 72°C 1min;	Hagen et al., 2012b
	GPD1 LR	5′ GGAGCGGAAATGACGACCTTCTT 3′	72°C 5min
IGS1	IGS1F	5′ CAGACGACTTGAATGGGAACG 3′	96°C 5min; 35 cycles: 96°C 30s, 61°C 30s, 72°C 1min;	Bovers et al., 2008b
	IGS2R	5′ ATGCATAGAAAGCTGTTGG 3′	72°C 5min
LAC1	LAC1F	5′ GGCGATACTATTATCGTA 3′	96°C 5min; 35 cycles: 96°C 30s, 52°C 30s, 72°C 1min;	Bovers et al., 2008b
	LAC1R	5′ TTCTGGAGTGGCTAGAGC 3′	72°C 5min
PLB1	PLB1F	5′ CTTCAGGCGGAGAGAGGTTT 3′	96°C 5min; 35 cycles: 96°C 30s, 56°C 30s, 72°C 1min;	Litvintseva et al., 2006
	PLB1R	5′GATTTGGCGTTGGTTTCAGT 3′	72°C 5min
SOD1	JOHE7777	5′ TTCAACCACGAATATGTA 3′	96°C 5min; 35 cycles: 96°C 30s, 52°C 30s, 72°C 1min;	D'Souza et al., 2004
	JOHE7779	5′ AAGCCTCTCATCCATATCTT 3′	72°C 5min
URA5	URA5F	5′ ATGTCCTCCCAAGCCCTCGAC 3′	96°C 5min; 35 cycles: 96°C 30s, 63°C 30s, 72°C 1min;	Meyer et al., 2003
	URA5R	5′ TTAAGACCTCTGAACACCGTACTC 3′	72°C 5min