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. 2013 Sep 3;8(9):e73080. doi: 10.1371/journal.pone.0073080

Figure 3. Dynamin-independent macropinocytosis regulates an early stage of amylin monomer internalization in RIN-m5F cells.

Figure 3

Cells were treated with EIPA, CytD, Wort or Dyn for 1 hour followed by human amylin (10 µM) incubation for an additional 1 hour at 37°C. Dextran at 40µg/ml was then added for 30 minutes. Confocal microscopy (A) and whole cell analysis (B–C) revealed reduced internalization and increased PM accumulation of both monomers (B) and dextran (C) in the presence of the macropinocytotic inhibitors as compared to controls. **P<0.01, hA vs. hA/treatments and ##P<0.01, dextran vs. dextran/treatments, n = 9. On the contrary, there was no change in their cellular distributions when treated with Dyn. NS P>0.1, hA vs. hA/Dyn and NS P>0.1, dextran vs. Dextran/Dyn, n = 9. Significance established by ANOVA followed by Dunnett-Square test. Bar 5µm.