Table 2. Effect of substrate, copper concentration and culture mode on laccase activity (UL^-1).

Syringaldazine was used for monitoring laccase activity.

Substrate	SmC	SSC
Soybean Pod Husk + 0 mM Cu+2	4.46 ± 1.38 (23)**	12.55 ± 0.52 (13)**
Soybean Pod Husk + 0.5 mM Cu+2	84.93 ± 8.46 (23)†	20.28 ± 3.87 (21)
Soybean Pod Husk + 2 mM Cu+2	57.61 ± 3.70 (19)	137.85 ± 0.71 (18)†
Soybean Pod Husk + 5 mM Cu+2	44.92 ± 4.87 (23)	29.00 ± 4.07 (18)
Coffee Husk + 0 mM Cu+2	61.84 ± 1.76 (23)**	204.00 ± 5.96 (19)**,†
Coffee Husk + 0.5 mM Cu+2	137.63 ± 5.68 (23)	110.83 ± 9.40 (20)a,b
Coffee Husk + 2 mM Cu+2	123.04 ± 3.73 (23)b	102.78 ± 8.14 (15)b
Coffee Husk + 5 mM Cu+2	177.23 ± 3.72 (23)†	82.66 ± 8.82 (15)
Cedar Sawdust + 0 mM Cu+2	13.55 ± 0.95 (17)*	10.82 ± 0.53 (15)*
Cedar Sawdust + 0.5 mM Cu+2	22.72 ± 0.84 (17)	19.02 ± 0.02 (13)
Cedar Sawdust + 2 mM Cu+2	28.97 ± 1.27 (12)a	26.38 ± 0.68 (8)
Cedar Sawdust + 5 mM Cu+2	29.36 ± 1.53 (13) a,b,†	30.73 ± 1.52 (12)b,†

Data in parenthesis refers to the day where was observed the maximum laccase activity

^* No Statistical Difference, ^** Statistical Difference. (Evaluation of substrate effect on laccase activity for both culture modes, submerged (SmC) and semisolid (SSC), no copper induction considered)

Equal letters inside one agro-industrial waste group refers to No Statistical Difference; no letters refers to Statistical Difference inside the group. (Evaluation of copper effect on laccase activity for both SmC and SSC)

^† Maximum laccase activity for the used agro-industrial wastes either in SmC or SSC culture.