Table 3. Laccase activity during the purification of crude extracts resulted from T . pubescens culture on agro-industrial wastes under submerged (SmC) and semisolid (SSC) cultures.

	Soybean pod husk				Coffee husk				Cedar sawdust
	Protein (mg mL-1)	Volumetric Activity (UL-1)	Specific Activity (U mg-1 protein)	Recovery (%)	Protein (mg mL-1)	Volumetric Activity (UL-1)	Specific Activity (U mg-1 protein)	Recovery (%)	Protein (mg mL-1)	Volumetric Activity (UL-1)	Specific Activity (U mg-1 protein)	Recover (%)
	SmC
Crude Extract	0.869	528.88	0.608	100	0.414	1527.08	3.688	100	0.431	107.35	0.249	100
0.45 µm Filtration	0.736	465.49	0.632	88	0.334	1178.92	3.529	77	0.444	120.53	0.271	112
UF retentate	1.010	787.31	0.779	74	0.378	1952.63	5.165	64	0.510	259.05	0.507	120
Lac1	0.494	54.71	0.110	1	0.232	23.99	0.103	0.2	0.222	8.38	0.037	0.8
Lac2	0.310	206.61	0.666	4	0.116	1072.40	9.252	7	0.040	152.34	3.837	14
	SSC
Crude Extract	1.146	1649.66	1.439	100	0.596	1549.07	2.601	100	1.065	232.03	0.217	100
0.45 µm Filtration	0.776	1283.92	1.654	77	0.474	1157.36	2.439	75	0.806	258.24	0.320	111
UF retentate	0.973	3350.55	3.443	101	0.428	1617.50	3.779	52	1.134	511.01	0.450	110
Lac1	0.097	88.54	0.912	2	0.104	15.14	0.145	0.3	0.042	13.15	0.313	2
Lac2	0.168	489.88	2.915	10	0.131	822.98	6.282	18	0.072	161.62	2.244	23

740 ABTS was used to determine laccase activity.