Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Invest Dermatol. 2013 May 7;133(12):2732–2740. doi: 10.1038/jid.2013.220

Figure 2. Effects of Pg on Dsc reporter activities.

Figure 2

(a-b). ChIP assays demonstrating direct binding of Pg to the Dsc2 promoter in the presence of Lef-1. Note that Pg does not bind the Dsc3 promoter in the presence of Lef-1 (b) or TCF4 (data not shown). Input, chromatin used for immunoprecipitation; IgG, IP with unspecific IgG. (c-f) Reporter assays in keratinocytes. (c) Co-expression of Pg and Lef-1 dramatically increases Dsc2 reporter activity while all other combinations of Pg and TCF/Lef factors have no effect. (d) The Dsc3 promoter is activated in the presence of Pg. This activation is reversed in cells co-expressing Pg and TCF4 or Lef-1. (e) Dsc2 promoter activation by Pg and Lef-1 is dependent on the presence of a functional TCF/Lef binding site. (f) Pg over-expression and loss of a functional TCF/Lef binding site increase Dsc3 reporter activity to a similar extend. Note that expression is normalized to the baseline expression of the WT promoter (set to 1). (g-h) Endogenous Dsc gene expression in keratinocytes transfected with different combinations of Pg and TCF/Lef factors. Error bars, standard deviation. Stars indicate statistically significant results (p-value < 0.05).