Figure 3.

Ethanol consumption compartmentalizes PTPα in the synaptosomal fraction of the DMS of mice. Mice had continuous access to 10% ethanol for 21 d in a two bottle choice paradigm. At the end of the last 24 h ethanol drinking session, DMS tissue was collected and the protein levels of PTPα (top) and Fyn (middle) in synaptosomal fraction (A) and in the total homogenate (B) were measured. Control animals underwent the same paradigm but had access to water only. GAPDH immunoreactivity was used as an internal loading control. Histograms represent the mean ratio of PTPα or Fyn/GAPDH ± SEM, and data are expressed as the percentage of control (mice with access only to water). **p < 0.01 (two-tailed unpaired t test). n = 3.