Figure 6.

Downregulation of PTPα expression in the DMS of mice does not alter motor coordination, ethanol-induced LORR, and spontaneous locomotor activity in mice. Experiments were conducted at the end of the drinking experiments described in the legend of Figure 5. A, B, Rotarod test. On the first day of the experiment, the time to reach 180 s criterion was recorded (A). One day later, animals were administered ethanol (1.5 g/kg) and placed and latency to fall from the rotarod was recorded every 15 min (B). C, D, LORR test. Three days after the Rotarod test, mice were given 3.2 g/kg ethanol and the latency to (C) and duration of (D) the LORR were recorded. E, Knockdown of PTPα in the DMS of mice does not affect spontaneous locomotor activity. One week after the end of the LORR experiment, animals were tested for spontaneous locomotor activity. Mice were placed in locomotor activity chambers, and the distance traveled was recorded for 30 min. Data are presented as cumulative locomotor activity (cm) during the testing period. Data are mean ± SEM. A, B, n = 6–9 for each group. C, D, n = 6–8 for each group. E, n = 10–12 for each group.