Figure 2.

PDGFRα expression is similar between gray and white matter regions. Images taken from P9 cortex (A) and corpus callosum (B) immunolabeled for PDGFRα. Fluorescence intensity measurements taken from both the cortex and corpus callosum at different antibody concentrations (C) show similar PDGFRα immunofluorescence intensity in both areas, suggesting that PDGFRα expression is similar between gray and white matter (two-way ANOVA, Bonferroni post test). A.U., arbitrary units. Images taken from P4 (D, E) and P8 (F, G) slice cultures from gray and white matter regions of NG2creBAC:ZEG mice immunolabeled for PDGFRα (red), demonstrating similar expression levels of PDGFRα between the two regions and two ages. Green represents GFP fluorescence. Scale bars, 25 μm. Error bars indicate SD.