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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: J Am Chem Soc. 2010 Feb 10;132(5):1598–1605. doi: 10.1021/ja907777f

Figure 2.

Figure 2

Optical absorption spectra of the deoxy and CO-bound derivatives of CuBMb (a) and the CO titration curves of CuBMb (b), in the presence and absence of 64 μM Cu+. The solid lines in (b) are the best-fit curves based on a single CO binding site in CuBMb. The Kd of CO was determined to be 420 and 360 μM for the Cu+-free and Cu+-bound CuBMb, respectively. In both cases, full saturation was not achieved even at a CO concentration of 1 mM. The protein concentration was 3.1 μM.