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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: J Am Chem Soc. 2010 Feb 10;132(5):1598–1605. doi: 10.1021/ja907777f

Figure 3.

Figure 3

Resonance Raman spectra of CO-bound CuBMb in the presence and in the absence of 100 μM Cu+ (a) and the νFe-CO versus νC-O inverse correlation plot of the data (b). The top two spectra in (a) were obtained from Cu+-free CuBMb; the bottom spectrum was obtained from Cu+-bound CuBMb, in the presence of either 12C16O or 13C18O. The 12C16O - 13C18O difference spectrum was obtained from the Cu+-free sample. In the difference spectrum all the heme modes cancel out, while the remaining positive and negative bands are associated with 12C16O- and 13C18O-related modes, respectively. The data shown in the νFe-CO versus νC-O inverse correlation plot in (b) are taken either from this work or from refs 40, 52, and 53.