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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: J Am Chem Soc. 2010 Feb 10;132(5):1598–1605. doi: 10.1021/ja907777f

Figure 5.

Figure 5

CO binding kinetic traces of CuBMb in the presence of Cu+ (a) and plots of the rate constant (b) and amplitude (c) of the fast kinetic phase as a function of the CO concentration. The biexponential kinetic traces in (a) were obtained by following the changes in the deoxy band (435 nm) and in the CO-bound band (420 nm) by mixing 4 μM CuBMb with 50 μM CO in the presence of 100 μM Cu+ (final concentrations) in pH 7.4 buffer in a stopped-flow system. The rate constants in (b) were obtained from a single-exponential fit of the fast phase of the kinetic traces. The amplitudes in (c) were calculated as ΔOD435 nm – ΔOD420 nm of the fast phase of the kinetic traces.