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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: J Am Chem Soc. 2010 Feb 10;132(5):1598–1605. doi: 10.1021/ja907777f

Figure 7.

Figure 7

Kinetic traces obtained following photodissociation of NO from CuBMb in the absence (a) or presence (b) of 65 μM Cu+. The protein and NO concentrations were 10 μM and 1.7 mM, respectively. The reduced protein solution was prepared with 9.0 mM ascorbate. The solid curves are the single-exponential fits of the data. The insets show plots of the observed rate constants as a function of NO concentration. In the absence of Cu+, the bimolecular rate constant was determined to be 1.6 × 105 M−1 s−1 on the basis of the slope of the best-fit solid line. In the presence of Cu+, the rate (7.4 × 107 s−1) was independent of the NO concentration.