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. 2013 Sep 7;19(33):5485–5492. doi: 10.3748/wjg.v19.i33.5485

Figure 5.

Figure 5

Propofol inhibits constitutively active nuclear factor-κB in MIA-PaCa-2 cells. A: MIA-PaCa-2 cells were treated with 0, 10, 25, 50, or 100 μmol/mL propofol for 72 h and nuclear extracts were probed for nuclear factor-κB (NF-κB) binding to a DNA consensus sequence. Results of NF-κB DNA-binding activity by electrophoretic mobility shift assay (EMSA); B: MIA-PaCa-2 cells were treated with 100 μmol/mL propofol for 24, 48, or 72 h and nuclear extracts were probed for NF-κB binding to a DNA consensus sequence. Results of NF-κB DNA-binding activity by EMSA. Retinoblastoma protein in the nuclear extract was used as a loading control.