Fig. 6.

PAK is required for platelet calcium signaling. A: platelets treated with vehicle, 10 μM IPA-3 or 10 μM PIR 3.5 on a surface of fibrinogen in the absence or presence of thrombin (1 U/ml) in solution. B: replicate samples of platelets on a surface of fibrinogen, loaded with Oregon Green BAPTA1-AM analyzed for calcium spiking in the absence or presence of thrombin (1 U/ml) in solution. C and D: visualization and quantification of PS exposure of platelets pretreated with vehicle, 10 μM IPA, or 10 μM IPR 3.5 on a surface of fibrinogen with thrombin in solution visualized by annexin V-FITC staining. Results are representative of 4 separate experiments.