Fig. 2.

Effect of chronic alcohol feeding of rats on RF transporter (RFVT)-3 and RFVT-1 protein expression in intestinal brush-border membrane (BBM) and basolateral membrane (BLM) domains. Intestinal BBMV (A) and BLMV (B) proteins (80 μg) were isolated from chronic alcohol-fed rats (4 wk) and their pair-fed controls, and Western blotting was performed. BBMV and BLMV proteins were separated on NuPAGE 4–12% mini gel and electroblotted onto polyvinylidene difluoride (PVDF) membrane. Blots were incubated with rabbit polyclonal anti-RFVT-3 and -RFVT-1 primary antibodies and detected as described (37, 38). Samples were normalized relative to β-actin protein expression. Data are means ± SE of 3 separate samples from multiple sets of rats. *P < 0.01.