Table 1.

Combination of primers used to amplify the respective genes by real-time and semiquantitative PCR

Gene Name	Forward and Reverse Primers (5′–3′)
Real-time PCR Primers
rRFVT-1	AGCTACCTGTAGTGGTGA; CTCAGCCCCTGAACCA
rRFVT-3	TAAGGAAGATCACGGGCACCTC; GTCATCCAACTGGCCAACACAG
rβ-Actin	GTCAGGTCATCACTATCGGC; CATGGATGCCACAGGATTCC
hnRNA primers
rRFVT-1	CTTTAGGCCAGCTTCTTG; AAAATGCGAGTGGACAG
rRFVT-3	TGGTGTTCTGCCCTCAG; GGGCCTGTGTTTGTAGC
rβ-Actin	CTGCTCTTTCCCAGATGAG; CTCATAGATGGGCACAGTG

rRFVT, rat riboflavin transporter; hnRNA, heterogeneous nuclear RNA.