Fig. 1.

Oxidative phosphorylation in wild-type Ins2^+/+ (WT) and Akita^+/Ins2-derived β-cells. A: schematic of the mitochondrial function assay used to measure oxygen consumption rate (OCR) over time. B: WT and Akita^+/Ins2-derived β-cells were seeded at 60,000 cells/well in 24-well Seahorse plates. The following day, cell culture medium was replaced with low-buffered DMEM for 1 h at 37°C prior to measuring basal OCR and the effects of the mitochondrial inhibitors oligomycin (O), FCCP (F), and antimycin A (A) on OCR. C: the individual bioenergetic parameters were calculated based on the measurements taken in B. Results are means ± SE; n = 4–5/group. *P < 0.05 compared with WT β-cells. D: the OCR and extracellular acidification rate (ECAR) of WT and Akita^+/Ins2-derived β-cells were plotted against each other according to the final basal measurement shown in B.