Fig. 7.

Mitochondrial protein composition in WT and Akita^+/Ins2-derived β-cells. A: cell lysates from WT and Akita^+/Ins2-derived β-cells were prepared and resolved on SDS-PAGE gels, transferred to PVDF membranes, and subjected to immunoblot analysis for the following antibodies: citrate synthase, VDAC, complex I (39-kDa subunit), complex II (70-kDa subunit), complex III core protein 1, complex IV subunit I, and ATP synthase α- and β-subunits. B: Western blot loading was controlled for by protein staining, and band intensities were quantified using AlphaView SA software. Results are expressed as mean fold change AU over WT β-cells ± SE; n = 3/group. C: citrate synthase activity was determined and normalized to cellular protein, as described in materials and methods. Results are means ± SE; n = 3/group. *P < 0.05 compared with WT β-cells.