Fig. 6.

Propagation of an action potential is prevented by Foxo1 overexpression. A and D: representative confocal x-y images of a control fiber (A; n = 10, N = 3) and a fiber expressing Foxo1-GFP (D; n = 10, N = 3) stained with the voltage-sensitive dye di-8-ANEPPS. N indicates number of mice per condition, and n indicates number of fibers tested. Yellow lines in A and D indicate the location of the line scan used for B and C, and E and F, respectively. B, C, E, and F, bottom: graphs of the time course of di-8-ANEPPS fluorescence in response to field stimulation in the regions indicated by white dashed boxes. G and H: average change in di-8-ANEPPS fluorescence in control fibers (G) and fibers expressing Foxo1-GFP (H) in response to field stimulation of alternating polarity.