Fig. 2.

TREK-1 expression in telomerized pregnant human uterine smooth muscle cells (pHUSMC) and HEK293-hTREK-1 cells. Top: PCR amplification showing similar expression of human TREK-1 (hTREK-1) in cultured pHUMSC before (P5) and after (tel) telomerization (left) and similar expression of β-actin (right). NT, nontemplate control. Bottom: Western blot showing expression of hTREK-1 in telomerized pHUSMC (left) wild-type (wt) or stably expressing scrambled small interfering RNA (siRNA) or siRNA targeted to hTREK-1 and showing expression of human hTREK-1 in HEK293 cells (right) in untransfected [wild-type (wt)] cells or cells stably transfected with myometrial hTREK-1 (HEK293-hTREK-1). mw, Molecular weight.