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. 2013 Jun 26;305(5):F701–F713. doi: 10.1152/ajprenal.00030.2013

Fig. 10.

Fig. 10.

Immunohistochemistry and quantitative analyses demonstrating that hypokalemia increases type A intercalated cell size and GS expression. A: double immunolabel of GS (brown) with AE1 (blue) in collecting ducts of control and hypokalemic mice. In both control (left) and hypokalemic (right) kidneys, there was a one-to-one correlation between GS expression and AE1 expression, identifying GS-positive cells as type A intercalated cells. In response to hypokalemia, intercalated cells in both the CCD (top) and OMCDi (bottom) appeared to be hypertrophied and exhibited increased GS immunolabel. B: quantitative analysis of GS immunolabel intensity (in arbitrary units) in individual intercalated cells in the CCD and OMCDi. Hypokalemia induced significant increases in GS expression in type A intercalated cells in both the CCD and OMCDi. C: quantitative analysis of the type A intercalated cell profile area. Hypokalemia induced a significant increase in the OMCDi intercalated cell profile area. n = 4 hypokalemic kidneys and 4 control kidneys for each comparison. *P < 0.05.