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. 2013 Jun 26;305(5):F628–F640. doi: 10.1152/ajprenal.00210.2013

Fig. 1.

Fig. 1.

Western blot analysis of organelle-specific proteins in the initial lysates of isolated proximal convoluted tubules (PCT) and soluble cytosolic fractions. Cathepsin B, aconitase 2, binding immunoglobulin protein (BiP), and cAMP response element binding protein 1 (CREB-1) are soluble proteins that are contained specifically in lysosomes, mitochondria, endoplasmic reticulum, and nuclei, respectively. Levels of GAPDH were also quantified to assess the recovery of a cytosolic protein.