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Western blot analysis of organelle-specific proteins in the initial lysates of isolated proximal convoluted tubules (PCT) and soluble cytosolic fractions. Cathepsin B, aconitase 2, binding immunoglobulin protein (BiP), and cAMP response element binding protein 1 (CREB-1) are soluble proteins that are contained specifically in lysosomes, mitochondria, endoplasmic reticulum, and nuclei, respectively. Levels of GAPDH were also quantified to assess the recovery of a cytosolic protein.
