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NKCC2 expression and phosphorylation at the macula densa. Sections from AMPK-β1−/− mice or WT control mice fed the ND or SD for 7 days were costained for NKCC2 (A) or NKCC2 pThr100/105 (B) (green) and for Tamm-Horsfall protein (THP; red). Macula densa cells (*) were identified as NKCC2-positive, THP-negative cells juxtaposed to a glomerulus (G).
