Fig. 7.

Expression and phosphorylation of distal Na⁺ transporters in ACC1-KI^S79A mice. ACC1-KI^S79A mice and littermate control mice were fed the ND or SD for 7 days. A: representative Western blots of whole kidney protein lysates from ACC1-KI^S79A mice and littermate WT control mice that received the ND or SD. Phosphorylation of NKCC2 on Thr^100/105 and NCC on Thr⁵⁸ was determined using a phosphospecific antibody. B–F: densitometric analysis of Western blots of mice on the ND and SD. n = 4–7. *P < 0.05 and **P < 0.001 for mice on the SD relative to mice on the ND; #P < 0.05 for ACC1-KI^S79A mice relative to WT mice on teh equivalent diet.