Fig. 4.

Silybin inhibits superoxide generation in diabetic mice kidney cortex and decreases the Nox4 protein expression. Measurement of superoxide generation in kidney cortex of 16-wk-old FVB mice, OVE26 mice, and OVE26 mice treated with silybin 100 mg/kg ip for 6 wk. A: bar graph of the percent increase in 2-OH-E⁺ generation in kidney cortex (means ± SE; n = 4 per group). **P < 0.01 FVB vs. OVE26; #P < 0.05 OVE26 vs. OVE26 + silybin. B: representative immunoperoxidase staining images of Nox4 protein in kidney sections from FVB (nondiabetic control), OVE26 (diabetic control), and OVE26 + silybin mice (diabetic treated).