Table 1. Details of the specific horse-radish peroxidase (HRP) labeled oligonucleotide probes used in this study, together with the unlabeled competitor and helper (H-) probes.
Probe | Sequence (5′–3′) | Specificity | E.coli target position | Label | Reference |
---|---|---|---|---|---|
EUB338-I | GCTGCCTCCCGTAGGAGT | Eubacteria | 16S rRNA 338–355 | HRP | 62 |
NON-EUB338-I | ACTCCTACGGGAGGCAGC | Eubacteria Negative control | 16S rRNA 338–355 | HRP | 63 |
BET42a | GCCTTCCCACTTCGTTT | Betaproteobacteria | 23S rRNA 1027–1043 | HRP | 64 |
BET42a competitor | GCCTTCCCACATCGTTT | Gammaproteobacteria | 23S rRNA 1027–1043 | – | 64 |
ARCH915 | GTGCTCCCCCGCCAATTCCT | Archaea | 16S rRNA 915–934 | HRP | 65 |
BET467 | CGTCATGGACGCGGCCTG | C. crambe Betaproteobacteria | 16S rRNA 467–484 | HRP | This study |
H-485 | GGCGCTTCTTCTCAGGGTAC | Helper | 16S rRNA 485–504 | – | This study |
H-446 | TTCGCCCGCGTTTTTTCTTTC | Helper | 16S rRNA 446–466 | – | This study |