Fig. 2.

D52 does not translocate to apical plasma membrane following acinar stimulation. All images are D52 localization following 2-min CCK-8 stimulation. A: D52 is on the cytoplasmic side of apical actin filaments (arrowheads) and absent from plasma membrane. Minimal colocalization of D52 is seen with the zymogen granule (ZG) protein VAMP2, which accumulates within actin filaments and along the apical membrane (arrows). Box in bottom left panel shows apical D52 that it is actually at the bottom of the acinus rather than on apical membrane (Supplemental Movie S1). B: D52 partially colocalizes with the recycling endosomal protein Rab11a on the cytoplasmic side of actin filaments. Dashed arrow indicates D52 and Rab11a above the plane of the actin filaments. Dashed lines in bottom panels designate enlarged areas. Scale bars, 7 μm. Images are reconstructed z-series from a representative experiment performed on 3 separate tissue preparations.