Fig. 7.

Characterization of the constitutive-like (CLP) and minor-regulated (MRP) pathways by LAMP1 externalization in freshly prepared acini. A: freshly prepared acini were treated as control or with CCK-8 (100 pM) for 5 min. Where indicated, acini were preincubated with BFA (10 μg/ml) for 30 min followed by CCK-8 (100 pM) for 5 min. Externalized LAMP1 labeling was conducted as in Fig. 5. Each image is a reconstructed z-series and representative of multiple determinations from at least 3 separate tissue preparations. Bars, 10 μm. B: quantitative analysis of the volume of LAMP1 immunoreactivity in acini acquired from multiple reconstructed z-series images of acinar clusters from 3 separate tissue preparations. Data are means and SE (n = 8 for each experimental condition). C: cells were processed as in A except they were costained with LAMP1 and syt1 and presented here in a reconstructed 3D format using Volocity software. Blue, red, and green arrows indicate direction in the z, y, and x planes, respectively. Con, control.