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Acute effect of U-46619 (2 μM) on superoxide production as indicated by dihydroethidium fluorescence in isolated afferent arterioles from WT mice (●; n = 6). U-46619 was added at 30 s. Preexposure of arterioles to tempol (▼; n = 6) eliminated the response to U-46619, which was indistinguishable from the time-control series given PBS vehicle rather than the thromboxane A2 mimetic (△; n = 6).
