Table 1. GAP activity is required for full EAT-17 function.
No. rescued | Total no. examined | % rescue | |
---|---|---|---|
Wild-type eat-17 construct | 16 | 76 | 21 |
R116K | 2 | 49 | 4.1 |
R116A | 1 | 64 | 1.6 |
eat-17 mutants were injected with DNA constructs encoding either wild-type or one of two mutant versions (R116K or R116A) of the EAT-17 protein (see Materials and Methods). F1 transgenic progeny were blindly assayed for rescue of grinder defects. GFP was used to identify transgenic progeny afterward.