Table 2. RNAi of rab-6.2 phenocopies eat-17 mutants.
| dsRNA injected | Phenotype |
|---|---|
| None | Wild type |
| rab-1 | Embryonic lethal |
| rab-2 | Slight growth delay |
| rab-3 | Wild type |
| rab-5 | Embryonic lethal |
| rab-6.1 | Wild type |
| rab-6.2 | Growth delay with abnormal grinder |
| rab-7 | Wild type |
| rab-8 | Loopy movement, exaggerated body bends |
| rab-10 | NDa |
| rab-11.1 | Embryonic lethal |
| rab-11.2 | Arrested as L1s |
| rab-14 | Some show growth delay, Dpyb |
| rab-18 | Growth delay, infrequent pumping but normal grinder |
| rab-19 | Wild type |
| rab-21 | Sick, starved, and strong Dpy |
| rab-27 | Wild type |
| rab-28 | Wild type |
| rab-30 | Wild type |
| rab-33 | Slightly Uncc, otherwise wild type |
| rab-35 | Wild type |
| rab-37 | Loopy movement, otherwise wild type |
| rab-39 | Wild type |
| 4R79.2 | Loopy movement, Unc, otherwise wild type |
| K02E10.1 | ND |
| F11A5.4 | Wild type |
| F11A5.3 | Wild type |
| C56E6.2 | Loopy movement, Unc |
Among 25 Rabs tested, RNAi of rab-6.2 shows an identical grinder phenotype to eat-17. Full-length dsRNAs for each Rab listed were injected into rrf-3 RNAi hypersensitive mutants. Worms were grown at 15° to enhance the phenotype.
a
ND, Not determined.
b
Dpy, dumpy phenotype, worms are shorter than wild type.
c
Unc, uncoordinated movement phenotype, worms move abnormally.