Table 2. Fitnesses and equilibrium frequencies of amino acids in buried and exposed sites in proteins.

	Exposed		Buried
Amino acid	πJ,buried	FJ,buried	πJ,exposed	FJ,exposed
D	0.0214	0	0.0871	0
E	0.0151	−0.348	0.0931	0.067
R	0.0202	−0.061	0.0646	−0.299
K	0.0076	−1.041	0.1021	0.159
H	0.0403	0.633	0.0480	−0.595
L	0.0806	1.326	0.0180	−1.576
M	0.0743	1.244	0.0225	−1.352
I	0.0831	1.356	0.0165	−1.663
V	0.0793	1.301	0.0225	−1.352
F	0.0781	1.294	0.0180	−1.576
W	0.0693	1.174	0.0150	−1.758
Y	0.0567	0.973	0.0225	−1.352
S	0.0416	0.663	0.0661	−0.276
T	0.0441	0.722	0.0586	−0.397
N	0.0264	0.211	0.0811	−0.072
Q	0.0214	0	0.0811	−0.071
C	0.0932	1.471	0.0091	−2.269
G	0.0504	0.856	0.0661	−0.276

Note that amino acid frequencies are taken from Table 1 in Baud and Karlin (1999). The fitnesses are calculated by solving Equation 16 and setting the fitness of asparagine (D) to zero so that F_J,k = log(π_J,k/π_D,k).