Table 1. Summary of major public Drosophila and Caenorhabditis elegans RNAi reagent collections.
| RNAi collection | Reagent type | All reagents | Target 1 gene, all isoforms | Target 1 gene, not all isoforms | Target multiple genes | Reagents with >5 OTEs (19 bp) | Target pseudogene | No gene target |
|---|---|---|---|---|---|---|---|---|
| DKFZ | dsRNA, cell based | 20,016 | 15,948 (80%) | 816 (4%) | 1466 (7%) | 394 (2%) | 78 (0.4%) | 1708 (9%) |
| DRSC-Genome Library | dsRNA, cell based | 24,037 | 19,615 (82%) | 1664 (7%) | 979 (4%) | 552 (2%) | 84 (0.4%) | 1695 (7%) |
| DRSC-Followup Library | dsRNA, cell based | 9448 | 8519 (90%) | 470 (5%) | 296 (3%) | 15 (0.2%) | 14 (0.2%) | 149 (2%) |
| NIG | dsRNA, transgenic fly | 11,725 | 10,328 (88%) | 532 (5%) | 436 (4%) | 416 (4%) | 2 (0.02%) | 427 (4%) |
| TRiP-LongHairPin | dsRNA, transgenic fly | 2483 | 2255 (91%) | 114 (5%) | 72 (3%) | 8 (0.3%) | 2 (0.1%) | 40 (2%) |
| TRiP-ShortHairPin | shRNA, transgenic fly | 4132 | 3738 (90%) | 242 (6%) | 120 (3%) | 0 (0%) | 2 (0.1%) | 30 (1%) |
| VDRC-GD Library | dsRNA, transgenic fly | 21,808 | 18,607 (85%) | 962 (4%) | 1357 (6%) | 745 (3%) | 60 (0.3%) | 822 (4%) |
| VDRC-KK Library | dsRNA, transgenic fly | 10,748 | 9135 (85%) | 431 (4%) | 378 (4%) | 67 (1%) | 27 (0.3%) | 777 (7%) |
| Ahringer Library | dsRNA, worm feeding | 16,256 | 11,002 (68%) | 678 (4%) | 1733 (11%) | 1074 (7%) | 283 (2%) | 2843 (16%) |
DKFZ, German Cancer Research Center; DRSC, Drosophila RNAi Screening Center; NIG, National Institute of Genetics (Japan); TRiP, Transgenic RNAi Project; VDRC, Vienna Drosophila RNAi Center.