Figure 2.

Downregulation of atr1 and chk1 resulted in an increase in abnormal mitosis in the dikaryon. (A) Cell images from the indicated strains. Septa with associated clamp connections are marked with arrowheads, while septa without clamp connections are marked with arrows. Nuclei are marked with asterisks. Dots mark the presence of nuclei trapped by a clamp connection. Bar: 20 μm. (B) Examples of cells resulting from abnormal mitosis. In B1, a cell compartment is flanked by a septum with no associated clamp connection (arrow) and five nuclei (asterisk). In B2, nuclei are trapped inside the clamp connection (asterisk). Bar: 10 μm. (C) Quantification of the number of aberrant mitoses (cell compartments with clamp-free septa and/or more than two nuclei) as well as clamps with trapped nuclei inside in hyphae from the indicated strains. The graph shows the results from three independent experiments, counting more than 100 cell compartments each. Means and standard deviations are shown.