Figure 6.

Axonal transport of Brp is impaired in unc-104^bris/− larvae. (A and A′) Ventral nerve cords of mid-third instar Drosophila larvae were stained for Brp, which is concentrated in the neuropil region (green N) of wild-type larvae, but is found in the cortex of unc-104^bris/− larvae (green C). (A′) Panneuronal expression of Unc-104^mCherry (rescue) restores Brp neuropil localization in unc-104^bris/− larvae. Bar, 20 µm. (B) NMJ 4, segment A2 of mid-third instar Drosophila larvae of the following genotypes: elav-Gal4/+;;UAS-unc-104^mCherry/+ (control), unc-104^bris/−;UAS-unc-104^mCherry/+ (unc-104^bris/−), or elav-Gal4/+;unc-104^bris/−;UAS-unc-104^mCherry/+ (rescue) were stained for Brp. Bar, 10 µm. (C) Brp density and (D) Brp abundance per NMJ are reduced in unc-104^bris/− larvae compared to control larvae and unc-104^bris/− larvae expressing unc-104^mCherry. The SEM is shown as a box, the SD as a black line. *P < 0.05, **P < 0.01, ***P < 0.001.