Figure 7.

Brp overexpression does not rescue the AZ and PSD apposition defect in unc-104^bris/− larvae. (A) Confocal images of neuromuscular synapses immunostained with Brp (magenta and gray) and GluRIIC (green) in larvae of the following genotypes: elav-Gal4/+;; (control), elav-Gal4/+;unc-104^bris/− (unc-104^bris/−), and elav-Gal4/+;unc-104^bris/−;UAS-Brp/+ (unc-104^bris/−,Brp↑). Arrows indicate PSDs unapposed by presynaptic Brp punctae. No Brp punctae without an apposed PSD were detected. Bar, 1 µm. Quantification of the abundance of Brp per NMJ (B), Brp quantity at a single puncta (C), and the percentage of PSDs unapposed by Brp (D). Experiments were performed at 29 °. The SEM is shown as a box, the SD as a black line. *P < 0.05, **P < 0.01.