Table 1.
Primer sequences for real-time PCR
| Gene | Forward Primer | Reverse Primer | Product Size, bp | Accession No. |
|---|---|---|---|---|
| GAPDH | TGACTCTACCCACGGCAAGTTCAA | ACGACATACTCAGCACCAGCATCA | 141 | XM_001062726.2 |
| Renin | CTGCCACCTTGTTGTGTGAG | ACCTGGCTACAGTTCACAACG | 154 | NM_012642.4 |
| AGT | TCCCTCGCTCTCTGGACTTA | AAGTGAACGTAGGTGTTGAAA | 209 | NM_134432.2 |
| AT1-R | CTCAAGCCTGTCTACGAAAATGAG | GTGAATGGTCCTTTGGTCGT | 188 | NM_030985.4 |
| AT2-R | ACCTTTTGAACATGGTGCTTTG | TTTCCTATGCCAGTGTGCAG | 160 | NM_030985.4 |
| ACE1 | GTGTTGTGGAACGAATACGC | CCTTCTTTATGATCCGCTTGA | 187 | AF539425.1 |
| ACE2 | TTAAGCCACCTTACGAGCCTC | GCCAATGTCCATGGAGTCAT | 170 | GQ262788.1 |
| Mas-R | TGTGGGTGGCTTTCGATT | CCCGTCACATATGGAAGCAT | 159 | NM_012757.2 |
| gp91phox | CAAGATGGAGGTGGGACAGT | GCTTATCACAGCCACAAGCA | 170 | AF298656.3 |
| ERα | CATCGATAAGAACCGGAGGA | TCTGACGCTTGTGCTTCAAC | 128 | AB477039 |
| ERβ | GAAGCTGAACCACCCAATGT | CAATCATGTGCACCAGTTCC | 112 | AB190770 |
AGT, angiotensinogen; AT-R, angiotensin receptor; ACE, angiotensin-converting enzyme; ER, estrogen receptor.