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. 2013 Jun 28;305(5):H699–H705. doi: 10.1152/ajpheart.00193.2013

Table 1.

Primer sequences for real-time PCR

Gene Forward Primer Reverse Primer Product Size, bp Accession No.
GAPDH TGACTCTACCCACGGCAAGTTCAA ACGACATACTCAGCACCAGCATCA 141 XM_001062726.2
Renin CTGCCACCTTGTTGTGTGAG ACCTGGCTACAGTTCACAACG 154 NM_012642.4
AGT TCCCTCGCTCTCTGGACTTA AAGTGAACGTAGGTGTTGAAA 209 NM_134432.2
AT1-R CTCAAGCCTGTCTACGAAAATGAG GTGAATGGTCCTTTGGTCGT 188 NM_030985.4
AT2-R ACCTTTTGAACATGGTGCTTTG TTTCCTATGCCAGTGTGCAG 160 NM_030985.4
ACE1 GTGTTGTGGAACGAATACGC CCTTCTTTATGATCCGCTTGA 187 AF539425.1
ACE2 TTAAGCCACCTTACGAGCCTC GCCAATGTCCATGGAGTCAT 170 GQ262788.1
Mas-R TGTGGGTGGCTTTCGATT CCCGTCACATATGGAAGCAT 159 NM_012757.2
gp91phox CAAGATGGAGGTGGGACAGT GCTTATCACAGCCACAAGCA 170 AF298656.3
ERα CATCGATAAGAACCGGAGGA TCTGACGCTTGTGCTTCAAC 128 AB477039
ERβ GAAGCTGAACCACCCAATGT CAATCATGTGCACCAGTTCC 112 AB190770

AGT, angiotensinogen; AT-R, angiotensin receptor; ACE, angiotensin-converting enzyme; ER, estrogen receptor.