Fig. 8.

Transduction of endothelial cells with plasma membrane-targeted Arg I elicits equal eNOS inhibition compared with WT Arg I. BAEC were transduced with 20 MOI of RFP, catalytically inactive Arg I (D128G), WT Arg I, and PM-targeted arginase (PM Arg I) adenoviruses. A, top: NO release from BAEC was measured by NO-specific chemiluminescence and presented as %NO release from cells transduced with RFP control. A, bottom: expression of relevant proteins was determined by immunoblotting. Hsp90 was used as a loading control. Nonparametric 1-way ANOVA and Kruskal-Wallis test with Dunn's multiple comparison. Results are represented as means ± SE; n = 6 experiments. *P < 0.05 vs. RFP. B: confocal microscopy of BAEC transduced with PM Arg I shows the predominant PM location of targeted arginase. Green indicates the FLAG tag (PM Arg I), and blue indicates DAPI (nucleus). Results are representative of more than 3 independent experiments.