Fig. 1.

Response of murine hearts with varying degrees of hypoxia-inducible factor-1α (HIF-1α) expression to 30 min of ischemia, followed by reperfusion. Shown are exemplary condensed pressure tracings from Langendorf-perfused hearts derived from wild-type (WT) and HIF-1α-PPN mice that were not induced (Non-I) and induced to express HIF-1α for 2 days (2D induced) or 6 days (6D induced). Hearts were subjected to 30 min of ischemia following preequilibration for at least 20 min, whereupon they were allowed to recover. In some hearts, the left ventricular balloon was deflated during ischemia and reperfusion. In the example shown here, the balloon was allowed to remain inflated so that ischemic contraction could be measured. LVDP, left ventricular developed pressure.