Fig. 1.

Cardiac-specific Mammalian enabled (Mena) transgenic mouse model. A: simplified schematic of constructs for creation of cardiac-specific Mena overexpression mouse model. MHC, myosin heavy chain; TetO, tetracycline operon; TetR, tetracycline resistance operon; TTA, tetracycline transactivator; VP16, virion protein 16. B: representative genotyping results showing PCR products generated by amplification of DNA extracted from mice in the TTA/TgTetMena colony. TTA primers were used to produce the bands in the gel on left, and TgTetMena primers were used to produce the bands in the gel on right. The lanes in each gel correspond to the same mouse, such that only lane 3 is positive for TTA/TgTet Mena.