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. 2013 Jul 5;305(6):H875–H884. doi: 10.1152/ajpheart.00342.2013

Fig. 7.

Fig. 7.

Reducing cardiac Mena overexpression immediately after injury does not protect against HF. A: representative immunoblot of whole cell lysates from LV tissue of TTA/TgTetMena mice given varying doses of doxycycline (Dox) in drinking water for 7 days. Lysates were separated by polyacrylamide gel electrophoresis and probed with antibodies to Mena and GAPDH. Lane on far right contains protein from LV of a WT animal, demonstrating that our dose of 0.5 mg/ml doxycycline is sufficient to restore WT levels of cardiac Mena expression in TTA/TgTetMena mice. B and C: summary echocardiography data showing ejection fraction (B) and fractional shortening (C) of sham-operated, WT, and TTA/TgTetMena mice given 0.5 mg/ml doxycycline in drinking water (††P ≤ 0.01, †††P ≤ 0.001 vs. sham operated; *P ≤ 0.05, **P ≤ 0.01 vs. WT by 2-way ANOVA with Bonferroni posttest; n = minimum 4 animals/group). D: Kaplan-Meier curve showing animal survival after TAC injury.