Figure 10.
Fractin immunostaining of injured axons in the cingulate grey matter. (A, B) Neurolucida computer renderings of fractin-stained axonal fragments (blue dots) are shown for coronal sections of the left cingulum bundle (CB) region with hemorrhage ([Hem], A) and the contralateral right CB region without hemorrhage (B). (C-F) A fractin stain from the region outlined with a red rectangle in (A) is shown at 20x power in (C) and 40x power in (E); a fractin stain from the region outlined with a red rectangle in (B) is shown at 20x power in (D) and 40x power in (F). The border between the white matter (WM) and cortex is indicated by the dashed white lines in (C, D), and fractin-stained axonal fragments are indicated by arrows in (C-F). Fractin-positive axonal fragments are seen within the cingulate cortices both ipsilateral and contralateral to the hemorrhage. They cluster in the deep laminae at the crests and sides of gyri, with sparing of the depths of the sulci.